Hand-segmented 2D Nuclear Images

The images available below are described in

L. P. Coelho, A. Shariff, and R. F. Murphy (2009)
Nuclei Segmentation In Microscope Cell Images: A Hand-Segmented Dataset And Comparison Of Algorithms.
Proceedings of the 2009 IEEE International Symposium on Biomedical Imaging (ISBI 2009), pp. 518-521.

Download images (170 MB tar/gzipped file)

The file contains original images from two microscopes for two cell types stained with Hoechst 33342 as PNG files. It also contains images (as Photoshop and GIMP files) showing hand-segmentation of the Hoechst images into regions containing single nuclei. It also contains the source code used for the paper.

Murphy Lab
Last modified: July 8, 2009