The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.

4:30-6:00 Session 1 - IMAGE SOFTWARE - Ken Castleman

6:00-7:00 DINNER

7:30-9:00 Session 1, continued

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 1, continued

10:00-10:30 BREAK

10:30-12:00 Session 2 - FLOW SOFTWARE - Jim Wood

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 3 - IMAGE HARDWARE - Ted Young

6:00-7:00 DINNER

7:30-9:00 Session 3, continued

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 4 - FLOW HARDWARE - Bob Hoffman

10:00-10:30 BREAK

10:30-12:00 Session 4, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 4, continued

6:00-7:00 DINNER

7:30-9:00 Session 5 - COMMON ISSUES - Stefan Miltenyi

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-9:00 UNFINISHED BUSINESS - Howard Shapiro

9:00-10:30 BREAK-OUT SESSIONS

10:30-12:00 BREAK-OUT SESSIONS

12:00-1:00 LUNCH

1:00-6:00 FREE TIME

6:00-7:00 DINNER

7:30-9:00 BREAK-OUT SESSION REPORTS

9:00-10:00 THE FUTURE

10:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

Departure

** = Tutorial; 15 minutes max.

* = Regular Presentation; 8 minutes max

Session 1 - IMAGE SOFTWARE - Ken Castleman

** Color Image Processing - Ken Castleman

* Image distortion in conventional and confocal microscopy - Ted Young

* Deblurring images - Mark Schulze

* Model-based resolution: applying the theory in quantitative microscopy - Ted Young

* Image segmentation under bad conditions - Martin Büscher

* Typical image selection - Bob Murphy

* Towards metrics to describe 3D biological structures - Damir Sudar

* Wavelet transform design - Ken Castleman

* Microarray analysis for gene expression research - Laura Kegelmeyer

* Quantitative texture analysis of fluorescently-labeled nuclear membrane protein as a marker for cancer in human breast tissue - David Knowles

* Classification of protein localization patterns - Bob Murphy

* Analysis of the 3D spatial organization of cells and subcellular structures in tissue - David Knowles

* Temporal area maps and histograms of cell motility - George McNamara

* Fetal cell detection using brightfield microscopy - Fatima Merchant

* Shape and color descriptors of cells on slides - Ilya Ravkin

* Elliptical Fourier analysis of cell shape - George McNamara

* Calcium Signaling Measurements - Anil Tarachandani

Session 2 - FLOW SOFTWARE - Jim Wood

** Many color analysis - Mario Roederer

* Spectral compensation and data interpretation - Jim Wood

* Multiplexed bead assay using Excel - Ben Verwer

* Pipeline processing of flow data - Matt Ottenberg

* Caveats for interpreting log histograms - Jim Wood

* Percent positive enumeration - Bob Hoffman

* Data managment - Wayne Moore

* Progress towards a public domain FCS support library - Bob Murphy

Session 3 - IMAGE HARDWARE - Ted Young

* Flexible control of the Digital Micromirror Device (DMD) - Mark Corio

* Cell classification algorithms for microvolume fluorimetry - Ning Sizto

* Four-color microvolume laser scanning cytometry - Lou Dietz

* Spectral Karyotyping (24-color FISH) - George McNamara

* Spectral FISH (>5 color interphase FISH) - George McNamara

* Spectral Pathology (bright-field multi-color specimens) - George McNamara

* Four-color FISH with a 3-CCD color camera - Fatima Merchant

* Quantitative FISH imaging - Mark Schulze

* Biochip arrays based on fluorescence measurements - Ted Young

* 3D imaging with block face microscopy and serial sectioning - Damir Sudar

* Modular approach to optical microscopy automation - Ilya Ravkin

Session 4 - FLOW HARDWARE - Bob Hoffman

* Theory of polarization applied to light signals in a flow cytometer - Chip Asbury

* Method for measuring polarization of scatter and fluorescence in a flow cytometer - Jeanne Fehlauer

* Use of fiber optics in flow cytometry - Roel Kassies

* Solid state UV/violet lasers - Howard Shapiro

* New light sources for flow cytometry - Bob Hoffman

* Optimization for multi-color measurements in flow cytometry - Marty Bigos

* Simplified log amp and baseline restorer circuits for convential flow systems - Rob Habbersett

* Digital Data Acquisition System for flow cytometry - Jeff Rodriguez

* Digital pulse processing - Howard Shapiro

** Objective, standardized characterization of sorting - Dave Parks

** High speed sorting - Kit Snow

** Clinical cell sorting - Dennis Sasaki

* Flow sorting rates - Dick Stovel

* High speed sorting - Ger van den Engh

* Single cell sorting - Ger van den Engh

* Sorting or large particles and non-spherical cells - Kit Snow

* SortMaster - George Malachowski

** DNA Fragment Sizing and Analysis- Robb Habbersett

* Progress in DNA fragment sizing with a miniature apparatus - Rob Habbersett

* Requirements for high throughput bead-based assays - Dennis Sasaki

* Detection and analysis of low level antigens or other markers - Bob Hoffman

* Small flow cytometers - Howard Shapiro

** Standardization and fluorescence quantitation in flow cytometry - Bob Hoffman

* The consequences of polarization on standardization of flow cytometry measurements - Ger van den Engh

* Fluorescence standardization and quantitation using log vs. linear data - Jim Wood

Session 5 - COMMON ISSUES - Stefan Miltenyi

* Cytometry of secreting cells - Stefan Miltenyi

* Unique properties of rare-earth tags - Bob Leif

* FCS, XML and DICOM - Bob Leif