2005 CYTOMETRY DEVELOPMENT WORKSHOP:
SESSION TOPICS AND MODERATORS
(Thursday afternoon 4:30 - 6:00)
Bob Hoffman - Use of visible & UV LED's for flow cytometry excitation
Paul Johnson - LED Illumination in Flow Cytometry
Yong Chen - Compact near UV laser excitation source and its application in high throughput desktop sorter.
Yong Chen - A novel top-hat beam shaper & its application in flow cytometer
Martin Buescher - The MACS Quant Concept & Design
Masahiko Kanda - Flow sorter using a near UV laser and a fiber optic detection system
James Wood - Strategies for high speed flow cytometer pulse digitization
SESSION 2 – IMAGE CYTOMETRY & AUTOMATED MICROSCOPY (Jeff Price)
(Thursday evening 7:30 - 9:00)
Paul Johnson - High-Throughput-Axial Imaging Flow Cytometry with LED illumination
Paul Johnson - Imaging Flow Cytometry
Lori Krueger - Automated image cytometry
Juchang Hua - The Protein Subcellular Location Image Database (PSLID): shared image collections and open source automated analysis system
Anne Carpenter - The open-source CellProfiler cell image analysis software project
Anne Carpenter - Genome-wide screens for a variety of cell phenotypes using automated microscopy & image analysis
Jeff Price - Image Cytometry Detection of Ultra-Rare Cancer Cells in Blood
David Zahniser – Current status of Pap test automation
SESSION 3 – FLOW SPECTROSCOPY (John Nolan)
(Friday morning 8:30 - 9:40)
John Nolan - Fluorescence and Raman spectroscopy in flow
Greg Goddard - Spectral analysis flow cytometry
Dakota Watson - Raman Flow cytometry
David Basiji - Flow spectroscopy by time delay integration
Mark Naivar - Spectroscopic data acquisition in flow
Adam Treister - Software Implications of Flow Spectroscopy
Bob Hoffman - Spectroscopy & conventional flow applications
SESSION 4 – IMAGING (David Zahniser)
(Friday morning 9:40 – 10:00)
David Zahniser - LED die arrays in microscopy
Qiang Wu - Multiresolution Microscope Autofocusing
Qiang Wu - Extended Depth-of-Field Microscope Imaging
Michael Zahniser - A new segmentation method; High speed, low cost microscopy imaging
SESSION 4 – IMAGING (CONTINUED)
(Friday morning 10:30 - 12:00)
(Friday afternoon 4:30 – 6:00)
Kovit Pattanapanyasat - Affordable; Alternative; New cheap technologies for CD4 Testing
Tutorial – Kovit Pattanapanyasat - Evaluation of 2 new inexpensive flow cytometers for CD4 T-cells enumeration in resource poor settings
Bob Hoffman - BD's efforts in clinical testing HIV/AIDS in resource poor countries
Bob Hoffman - NIST fluorescence standardization program
David Parks- Quantification of antibody staining
Doug Redelman - Standardization of immunofluorescence measurements in flow cytometry
(Friday evening 7:30 - 9:30)
Howard Shapiro - Cellular astronomy
Bob Murphy - Building generative models from fluorescence microscope images for systems biology
Bart Vermolen - 3 dimensional spectral karyotyping
Jeff Price - 3D Cytometry for Drug and Toxicity Screening
Jeff Price - Unsupervised Spectral Decomposition for
Automated Densitometry in Histological Sections
Jeff Price - Membrane Segmentation for Generalized Image Cytometry
Damir Sudar – Exploratory visualization of information from images
Cris Luengo - Measuring expression patterns in fruitfly embryos
Hanchuan Peng - Image mining for fruitfly gene expression data
(Saturday morning 8:30 – 9:20)
Ge Yang - Probing spatial and temporal dynamics of cytoskeleton using automated large-scale particle tracking
Elliot Botvinick - Video rate sperm tracking with automated swimming force measurements and swimming characterization through object tracking and image analysis
Elliot Botvinick - Internet accessible laser scissor and laser tweezers
Elliot Botvinick - Multi-Level Thresholds for pixel class separation to study kinase activity with FRET probes
Elliot Botvinick - Ultra-localized fluid shear stresses on cell by rotating microspheres in optical traps
(Saturday morning 9:20 – 10:00)
Eric Chase - Q & B Measurement
David Parks - Instrument diagnosis using control samples & test particles
Marty Bigos -Optimizing filter selection for flow optometry
David Parks - Fully automated & optimized fluorescence compensation
(Saturday morning 10:30 - 12:00)
John Nolan - Small particle flow cytometry
Jakob Waldbauer - Flow Cytometry of marine bacterioplankton populations (cultured & natural)
James Freyer - Flow cytometry of isolated mitochondria
Tutorial – Nigel Llewellyn-Smith - The flow antibody market & the future of flow
Joseph Spidlen - A flow cytometry ontology
William Godfrey - Dyes for live cell analysis
(Saturday afternoon 4:30 - 6:00)
Ryan Brinkman - Bioinformatics Data Standards for Flow Cytometry
John Watkins - Using the client server model in flow analysis
Adam Treister - Statistical Comparison of Biological Populations
Aaron Perilatt - The availability and usage of a free Java package that allows the user to create software for reading and writing FCS sample files retrieved from a flow cytometer
Maciej Simm - Multicolor analysis and Polyvariate Display
(Saturday evening 7:30 - 9:00)
Tutorial – Richard Konz - High Content Polychromatic Flow Cytometry: HTS
Perry Haaland - Informatics issues for plate based flow
Charles Schmitt - Organizing gated results for analysis
Marty Bigos - Comparison of dead cell dyes for permeabilized cells
Stephen Perfetto - 17 color panel construction & cell viability
Stephen Perfetto - Advantages of the green laser in polychromatic flow cytometry