2004 CYTOMETRY DEVELOPMENT WORKSHOP:

SESSION TOPICS AND MODERATORS

 

SESSION 1 – CYTOMETRY STANDARDS AND APPLICATIONS (Howard Shapiro)

(Friday afternoon 4:30 - 6:00)

Bob Hoffman – Standardization for fluorescence in cytometry: update of NIST projects

Bob Hoffman – Characterizing bead standards for flow cytometry

Tutorial – Doug Redelman – The current status of immunofluorescence standards for flow cytometry

Doug Redelman – Immunofluorescence standards for flow cytometry

Tutorial – Howard Shapiro Cellular Astronomy

Howard Shapiro – Cellular Astronomy

Jan Greve – EasyCount: Affordable CD4+ enumeration

 

SESSION 2 – FLOW CYTOMETRY INSTRUMENTATION AND SORTING (Bob Auer)

(Friday evening 7:30 - 9:00, Saturday morning 8:30 - 10:00)

Robert Auer Data acquisition systems for FCM

Tutorial – Ed Stanton – Analog Digital hybridization

Ed Stanton – Analog Digital hybridization

Kit Snow – Digitization rate and measurement error in flow cytometry

David Parks – Investigation of noise levels and data quality limitations in systems with DiVa electronics

David Parks – FACSAria nozzle corrosion prevention, improvement of drop charging and enhancing deflection amplitudes

Richard Allman – Negative selection of ultra-rare events

Andrew Lewis – Sorting cells with all parameters stored and retrievable (indexed sorting)

Tutorial – Steve Perfetto – Infectious cell sorting: Containment procedures and operator safety

Steve Perfetto – Infectious cell sorting: Containment procedures and operator safety

 

SESSION 3 – IMAGE SOFTWARE AND APPLICATIONS (Bob Murphy)

(Saturday morning 10:30 - 12:00, Saturday afternoon 5:00 – 6:00,

Saturday evening 7:30 – 9:00)

Tutorial – Chris LuengoGranulometries: estimating size distribution of objects in images without segmentation

Chris Luengo – Imaging and quantifying 3D gene expression patterns in drosophila embryos

Chris Luengo – Segmentation of nuclei in 3D images of drosophila embryos

Tutorial – Peng Hanchuan – Image data mining of fruitfly gene expression patterns

Peng Hanchuan – Comparing 4D gene expression patterns of early fruitfly embryos

Tutorial – Bart Vermolen – Image Restoration: Processing for better Resolution

Bart Vermolen – 3D analysis of the distribution of telomeres in mammalian cells

Ram Sripad – Localization accuracy in single molecule microscopy

David Knowles – Understanding the early Drosophila transcription network at cellular resolution using high resolution fluorescence imaging and image analysis

Fuhui Long – Textural Image Analysis

Jeff Price – Functional Proteometrics with a RhoA GTPase FRET Biosensor

Jeff Price – Multi-Field Cell Tracking

Tutorial – Richard Levenson – Spectral imaging and unmixing: the right way, the wrong way and the CRI way

Richard Levenson – Spectral imaging in pathology

Jeff Price – Unsupervised Immunohistochemistry Multispectral Unmixing

Damir Sudar –

Bill Hyun –

 

SESSION 4 CYTOMETRY SOFTWARE (Nancy Perlmutter)

(Sunday morning 8:30 - 10:00)

David Parks – Update on Logicle data presentation

Dave Novo – Flow cytometry data display issues

James Wood – Understanding wide dynamic range flow data scaling

Adam Treister – MIFACE

Bob Murphy – Aligning cytometry annotations and data

James Wood – Future of data standards

Nancy Perlmutter – Future Cytometry Development Workshops

 

SESSION 5 MULTI COLOR FLOW CYTOMETRY (Bob Hoffman)

(Sunday morning 10:30 - 12:00, Sunday afternoon 5:00 - 6:00)

Bob Hoffman – LEDs for flow cytometry-update

Bob Hoffman – Multi-color immunofluorescence flow cytometry with a violet diode laser

Tutorial – George Malachowski – Spectral align mixing

George Malachowski – Spectral align mixing

Tutorial – Michael W. Olszowy – The Photo stability of the Alexa Fluor Dyes in Flow Cytometry and Microscopy

Tutorial – Steve Perfetto – Polychromatic flow cytometry: Instrumentation, panel selection acquisition and analysis

Steve Perfetto – Polychromatic flow cytometry: Instrumentation, panel selection acquisition and analysis

David Parks – Further data quality comparisons between instruments and lasers/laser powers