Thirteenth Cytometry Development Workshop |
Held with the endorsement of |
Co-chairs: Robert F. Murphy & Howard Shapiro |
Sponsored by: | Beckman Coulter, Inc. |
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No recordings of any session
No cameras during technical sessions
Time limit of 10 min. per presentation
Time limit of 15 min. per tutorial
The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.
WEDNESDAY
6:00-7:00 DINNER
7:30-9:00 Session 1 - FLOW HARDWARE, SOFTWARE AND APPLICATIONS
9:00-10:00 HAPPY HOUR / Informal Discussions
THURSDAY
7:30-8:30 BREAKFAST
8:30-10:00 Session 1, continued
10:00-10:30 BREAK
10:30-12:00 Session 1, continued
12:00-1:00 LUNCH
1:00-5:00 FREE TIME
5:00-6:00 Session 2 - IMAGE HARDWARE AND SOFTWARE
6:00-7:00 DINNER
7:30-9:00 Session 2, continued
9:00-10:00 HAPPY HOUR / Informal Discussions
FRIDAY
7:30-8:30 BREAKFAST
8:30-10:00 Session 3 - FLOW HARDWARE
10:00-10:30 BREAK
10:30-12:00 Session 3, continued
12:00-1:00 LUNCH
1:00-5:00 FREE TIME
5:00-6:00 Session 4 - IMAGE SOFTWARE AND APPLICATIONS
6:00-7:00 DINNER
7:30-9:00 Session 4, continued
9:00-10:00 HAPPY HOUR / Informal Discussions
SATURDAY
7:30-8:30 BREAKFAST
8:30-9:00 Compilation of Break-out session topics
9:00-10:15 BREAK-OUT SESSIONS
10:30-12:00 BREAK-OUT SESSIONS
12:00-1:00 LUNCH
1:00-6:00 FREE TIME
6:00-7:00 DINNER
7:30-10:00 BREAK-OUT SESSION REPORTS
9:00-10:00 HAPPY HOUR / Informal Discussions
THURSDAY
7:30-8:30 BREAKFAST
Departure
Tutorial: 15 minutes max, Regular Presentation: 10 minutes max
SESSION 1 - FLOW HARDWARE, SOFTWARE AND APPLICATIONS (Dave Coder)
Robb Habbersett - Advances and observations with DNA fragment sizing inflow: large and small fragments
Bill Godfrey - Getting dyes into gram-negative bacteria
Nancy Perlmutter - Is there a need for a low cost, open design flow cytometer?
Howard Shapiro - Cytometry for resource poor countries
Tutorial - Andrei Chernyshev - Dynamic Flow Cytometry
Andrei Chernyshev - Scanning Flow Cytometry for study of erythrocyte lysis kinetics
Andrei Chernyshev - Scanning Flow Cytometry for monitoring initial stage of immuno-agglutination
Andrei Chernyshev - study of evolution of cell distribution during ligand-receptor binding
Dave Coder - Amnis ImageStream system
Mario Roederer - Multicolor immunophenotyping: compensation, data analysis, data presentation.
Jim Wood - Effect of scaling on the display of wide dynamic range data
David Parks - Improved data display methods
Adam Treister - Flow Cytometry Markup Language
Adam Treister - FlowJo for Windows
Marty Bigos - Design of a data management system for flow and image cytometry
SESSION 2 - IMAGE HARDWARE AND SOFTWARE (David Zahniser)
Tutorial - Bob Murphy - Overview of current feature selection and classification methods
Bob Murphy - Consensus Subcellular Location Trees
Bob Murphy - Validating methods for comparing image sets
Tutorial - Elvira Garcia Osuna - MACE filters for image pattern matching
David Zahniser - LED's for microscope illumination
SESSION 3 - FLOW HARDWARE (Nancy Perlmutter)
Bob Hoffman - Practical issues in specifying and testing performance in a new cytometer - BD FACSArray Bioanalyzer
David Parks - Making valid comparisons among instruments
Marty Bigos- Possibly another comparison of instruments?
Bernard Verwer - Optical design in the FACSAria
Bernard Verwer - Cuvette sorting in the FACSAria
Bob Hoffman - Experience with APD detectors
Bob Hoffman - Status of new solid state UV light sources
David Parks - Optimizing multicolor signal quality
Bob Hoffman - Status of NIST fluorescence standards
Doug Redelman - Standardization of immunofluorescence using solution and bead-based approaches
Bob Auer - Data Acquisition Systems - Faster and more parameters
Kit Snow - At what point in the electronics of a flow cytometry should the signals be digitized?
SESSION 4 - IMAGE SOFTWARE AND APPLICATIONS (Bill Hyun)
Tutorial - David Zahniser - Update on Pap Test imaging automation
Bill Hyun - 3D image standards
David Knowles - Imaging method for recording 3D gene expression in Drosophila blastoderm embryos
David Knowles - The highly organized distribution of a nuclear protein is lost in malignant cells- This suggests NuMA-organization is a molecular signature of mammary cancer
Ramses Agustin - Expression-independent detection of cancer in an in vitro blood model
Damir Sudar - Quantifying the bystander effect after microbeam X-ray radiation
REPORTS/RECOMMENDATIONS