Thirteenth Cytometry Development Workshop
October 15-18, 2003
Asilomar Conference Center
Pacific Grove, California

Held with the endorsement of
ISAC, the International Society for Analytical Cytology
Co-chairs:
Robert F. Murphy & Howard Shapiro
Sponsored by: Beckman Coulter, Inc.
Overview of Program
Detailed Program
Reports/Recommendations
Past Workshops
1999
2000
2001
2002
Future Workshops
2004

General Rules of the Workshop

• No recordings of any session

• No cameras during technical sessions

• Time limit of 10 min. per presentation

• Time limit of 15 min. per tutorial

OVERVIEW OF PROGRAM

The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.

WEDNESDAY

6:00-7:00 DINNER

7:30-9:00 Session 1 - FLOW HARDWARE, SOFTWARE AND APPLICATIONS

9:00-10:00 HAPPY HOUR / Informal Discussions

THURSDAY

7:30-8:30 BREAKFAST

8:30-10:00 Session 1, continued

10:00-10:30 BREAK

10:30-12:00 Session 1, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 2 - IMAGE HARDWARE AND SOFTWARE

6:00-7:00 DINNER

7:30-9:00 Session 2, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

FRIDAY

7:30-8:30 BREAKFAST

8:30-10:00 Session 3 - FLOW HARDWARE

10:00-10:30 BREAK

10:30-12:00 Session 3, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 4 - IMAGE SOFTWARE AND APPLICATIONS

6:00-7:00 DINNER

7:30-9:00 Session 4, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

SATURDAY

7:30-8:30 BREAKFAST

8:30-9:00 Compilation of Break-out session topics

9:00-10:15 BREAK-OUT SESSIONS

10:30-12:00 BREAK-OUT SESSIONS

12:00-1:00 LUNCH

1:00-6:00 FREE TIME

6:00-7:00 DINNER

7:30-10:00 BREAK-OUT SESSION REPORTS

9:00-10:00 HAPPY HOUR / Informal Discussions

THURSDAY

7:30-8:30 BREAKFAST

Departure

DETAILED PROGRAM - TOPICS AND SPEAKERS

Tutorial: 15 minutes max, Regular Presentation: 10 minutes max

SESSION 1 - FLOW HARDWARE, SOFTWARE AND APPLICATIONS (Dave Coder)

Robb Habbersett - Advances and observations with DNA fragment sizing inflow: large and small fragments

Bill Godfrey - Getting dyes into gram-negative bacteria

Nancy Perlmutter - Is there a need for a low cost, open design flow cytometer?

Howard Shapiro - Cytometry for resource poor countries

Tutorial - Andrei Chernyshev - Dynamic Flow Cytometry

Andrei Chernyshev - Scanning Flow Cytometry for study of erythrocyte lysis kinetics

Andrei Chernyshev - Scanning Flow Cytometry for monitoring initial stage of immuno-agglutination

Andrei Chernyshev - study of evolution of cell distribution during ligand-receptor binding

Dave Coder - Amnis ImageStream system

Mario Roederer - Multicolor immunophenotyping: compensation, data analysis, data presentation.

Jim Wood - Effect of scaling on the display of wide dynamic range data

David Parks - Improved data display methods

Adam Treister - Flow Cytometry Markup Language

Adam Treister - FlowJo for Windows

Marty Bigos - Design of a data management system for flow and image cytometry

SESSION 2 - IMAGE HARDWARE AND SOFTWARE (David Zahniser)

Tutorial - Bob Murphy - Overview of current feature selection and classification methods

Bob Murphy - Consensus Subcellular Location Trees

Bob Murphy - Validating methods for comparing image sets

Tutorial - Elvira Garcia Osuna - MACE filters for image pattern matching

David Zahniser - LED's for microscope illumination

SESSION 3 - FLOW HARDWARE (Nancy Perlmutter)

Bob Hoffman - Practical issues in specifying and testing performance in a new cytometer - BD FACSArray Bioanalyzer

David Parks - Making valid comparisons among instruments

Marty Bigos- Possibly another comparison of instruments?

Bernard Verwer - Optical design in the FACSAria

Bernard Verwer - Cuvette sorting in the FACSAria

Bob Hoffman - Experience with APD detectors

Bob Hoffman - Status of new solid state UV light sources

David Parks - Optimizing multicolor signal quality

Bob Hoffman - Status of NIST fluorescence standards

Doug Redelman - Standardization of immunofluorescence using solution and bead-based approaches

Bob Auer - Data Acquisition Systems - Faster and more parameters

Kit Snow - At what point in the electronics of a flow cytometry should the signals be digitized?

SESSION 4 - IMAGE SOFTWARE AND APPLICATIONS (Bill Hyun)

Tutorial - David Zahniser - Update on Pap Test imaging automation

Bill Hyun - 3D image standards

David Knowles - Imaging method for recording 3D gene expression in Drosophila blastoderm embryos

David Knowles - The highly organized distribution of a nuclear protein is lost in malignant cells- This suggests NuMA-organization is a molecular signature of mammary cancer

Ramses Agustin - Expression-independent detection of cancer in an in vitro blood model

Damir Sudar - Quantifying the bystander effect after microbeam X-ray radiation

REPORTS/RECOMMENDATIONS