The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.

4:30-6:00 Session 1 - IMAGE HARDWARE AND SOFTWARE

6:00-7:00 DINNER

7:30-9:00 Session 1, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 2 - FLOW HARDWARE

10:00-10:30 BREAK

10:30-12:00 Session 2, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 3 - IMAGE SOFTWARE AND APPLICATIONS

6:00-7:00 DINNER

7:30-9:00 Session 3, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 4 - FLOW HARDWARE AND SOFTWARE

10:00-10:30 BREAK

10:30-12:00 Session 4, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 4, continued

6:00-7:00 DINNER

7:30-9:00 Compilation of Break-out session topics

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 BREAK-OUT SESSIONS

10:30-12:00 BREAK-OUT SESSIONS

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 BREAK-OUT SESSION REPORTS

6:00-7:00 DINNER

7:30-10:00 AT BILL HYUN'S BEACH HOUSE - THE FUTURE

7:30-8:30 BREAKFAST

Departure

Tutorial: 15 minutes max, Regular Presentation: 10 minutes max

SESSION 1 - IMAGE HARDWARE AND SOFTWARE (David Zahniser)

Mark Corio - Micromirrors and controllers, status update

David Zahniser - Autofocus using VCSELs

David Zahniser - LED illumination in microscopes

Jeff Price - Digital Micromirror device confocal microscopy

Howard Shapiro - New applications for LED-based imaging cytometers

Bill Hyun - Thin film standards for imaging/confocal QC

Tutorial - Ewert Bengtsson - Some thoughts on the need for well defined features in cytometry

Tutorial - Kai Huang - Feature reduction methods for automated classification

Kai Huang - Feature reduction and classifier choice for improved recognition of subcellular location patterns in fluorescence microscope images

Xiang Chen - Subcellular location pattern identification from high resolution 3D fluorescence images

Ewert Bengtsson - High speed segmentation and features extraction of fluorescent cell images

Robust, source-independent features for subcellular pattern analysis

Yu-Ping Wang - Chromosome classification: PCA versus LDA

Yu-Ping Wang - Spline based image interpolation

Damir Sudar - technical hurdles in building a system for bystander effect analysis after single-cell microbeam radiation by synchrotron

SESSION 2 - FLOW HARDWARE (Marty Bigos)

Bob Auer - Data acquisition systems for flow; Faster, More parameters

Ben Verwer - Update on FACSDiVa digital data acquisition system

Jeremy Hatcher - DSP in flow systems

Kit Snow - Do we need DSP in flow?

Jim Wood - Using photon statistics to predict instrument performance

Bob Hoffman - Generalizing the "Q+B" method of fluorescence detection capability to various detectors and fluorescence instrumentation

David Parks and Marty Bigos - Detailed flow cytometer evaluations and comparisons

Dick Stovel - Evaluating emission optics

Bob Hoffman - Trade-offs in selecting optical detectors for cytometric applications

Nancy Perlmutter - PMT and APD detectors for flow cytometry

Bob Hoffman - Update on UV, violet and blue solid state lasers for flow cytometry

John McInerney and Aram Mooradian - The Protera: a novel frequency doubled semiconductor laser for biotech applications

Valeri Maltsev - Pulsed laser excitation in a flow cytometer

SESSION 3 - IMAGE SOFTWARE AND APPLICATIONS (David Knowles)

Yu-Ping Wang - Digital image zoom and colorization

Yu-Ping Wang - Wavelet based chromosome image enhancement

David Knowles - Quantitative 3D imaging to analyze developmental variability in pregrastrula drosophila embryos

Tutorial - Dave Novo - Limitations of Ca++ probes for measuring fast (under 30 ms) Ca++ transients

Dave Novo - Optical studies of excitation-contraction coupling in mdx and normal mice

Tutorial - George Malachowski and Vicki Mosiman - Image processing in Immunohistochemistry

Susan Catalano - High content oncology assay development

Yu-Ping Wang - Deblurring and fusion of M-Fish images

Tutorial - Bob Murphy - PSLID and OME image databases

Susan Catalano - Establishment of an industrial high content screening facility

John McLaughlin - "Looking under the hood" of a high content oncology assay

Jeff Price - High throughput microscopy for drug screening

Jeff Price - Automated tissue microarray analysis

SESSION 4 - FLOW HARDWARE, SOFTWARE AND APPLICATIONS (Harald Steen)

Tutorial - Valeri Maltsev - Physics of light scattering

Harald Steen - Light scattering measurement of sub-microscopic particles/virus by flow cytometry

Valeri Maltsev - Do FS and SS signals help flow cytometrists to characterize a particle?

Valeri Maltsev - Scanning Flow Cytometry is the unique way for particle characterization

Valeri Maltsev - Flow Cytometry and Scanning Flow Cytometery <==> Colorimetry and Spectroscopy (light scattering domain)

Harald Steen - Flow cytometer fluidics

Topic for Discussion - Limiting factors for sensitivity in Flow Cytometry

Ben Verweer - Update on compensation of digital data

Dave Parks - Fluorescence compensation procedures and artifacts

Dave Parks - Improving displays of multi-color data

Jim Wood - Update on BNF formalization of FCS 3.x file format

Adam Treister - XML & Remote Compute Engine

Maciej Simm - Networking and storage of FCS (and other) data - RAID systems, etc.

Tutorial - Alan Waggoner - Fluorescent probes for flow and image cytometry

Richard Haugland - Antibody labeling technique

Doug Redelman - Quantitative immunofluorescence standards for flow cytometry

Doug Redelman - Individual variations in the quantitative expression of receptor/effector molecules of immunologically relevant cells

Nancy Perlmutter - Cellular analysis with the Luminex 100 instrument (green YAG and red diode lasers)

Rob Habbersett - DNA fragment sizing in flow

REPORTS/RECOMMENDATIONS