The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.

4:30-6:00 Session 1 - IMAGE HARDWARE

6:00-7:00 DINNER

7:30-9:00 Session 1, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 2 - FLOW HARDWARE

10:00-10:30 BREAK

10:30-12:00 Session 2, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 3 - IMAGE SOFTWARE

6:00-7:00 DINNER

7:30-9:00 Session 3, continued

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 4 - FLOW HARDWARE AND SOFTWARE

10:00-10:30 BREAK

10:30-12:00 Session 4, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 4, continued

6:00-7:00 DINNER

7:30-9:00 Compilation of Break-out session topics

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 BREAK-OUT SESSIONS

10:30-12:00 BREAK-OUT SESSIONS

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 BREAK-OUT SESSION REPORTS

6:00-7:00 DINNER

7:30-9:00 THE FUTURE

9:00-10:00 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

Departure

Tutorial: 15 minutes max, Regular Presentation: 10 minutes max

SESSION 1 - IMAGE HARDWARE (Meel Velliste)

Optical sectioning with structured light - Damir Sudar

TUTORIAL: Microscopy autofocus - Jeff Price

Single photon confocal: quantitative imaging or quagmire? - David Knowles

High Throughput Microscopy: Instrumentation Design for Fidelity & Speed - Jeff Price

High Throughput Microscopy: the Pick-and-Shovel of the Proteomics Era - Jeff Price

Basic principles of CellTracks, magnetic cell selection and aligning - Arjan Tibbe

Revisiting and imaging of specific events after first analysis with a scanning imaging technique implemented in the CellTracks setup - Arjan Tibbe

SESSION 2 - FLOW HARDWARE (Marty Bigos)

Peltier module based temperature control for flow cytometers - Steven Graves

Rapid sample delivery for flow cytometers - Steven Graves

Kinetic and mechanistic flow - John Nolan

TUTORIAL: Physics and chemistry of fluorescence - Howard Shapiro

Results with 400 nm Nichia diode laser - Bob Hoffman

Update on LEDs as excitation sources in flow - Bob Hoffman

Two-color photon-counting data acquisition - Rob Habbersett

Advances in DNA fragment sizing - Rob Habbersett

Multianode PMT's - Bob Hoffman

Digital data acquisition issues for flow - Bob Hoffman

Digital pulse processing, pro and con - Nancy Perlmutter

24-bit digital processing - Howard Shapiro

Digital electronics for the FACSDiVa: System Architecture - Ben Verwer

A real size measure in flow? - Howard Shapiro

What we did to our LSR - Dick Stovel

SESSION 3 - IMAGE SOFTWARE AND APPLICATIONS (Damir Sudar)

CellTracks based cell analysis - Leon Terstappen

Fidelity and Throughput in Screening for Drug Discovery - Jeff Price

Mapping organism expression levels at cellular resolution in developing Drosophila - David Knowles

Pap smear screening - David Zahniser

3D visualization of volume data - Damir Sudar

Building similarity trees for protein location - Bob Murphy

Object level recognition of protein subcellular location patterns - Meel Velliste

Automated recognition of subcellular patterns in 3D - Meel Velliste

Robust features for recognition of protein location patterns in images at different resolutions - Meel Velliste

Recognizing and interpreting fluorescence microscope images in online journals - Bob Murphy

Fluorescence microscope image database schema: results from preliminary implementations - Bob Murphy

Distributed imaging informatics through science portals - Damir Sudar

SESSION 4 - FLOW HARDWARE AND SOFTWARE (Nancy Perlmutter & Kit Snow)

Optimization of a flow cytometer for various fluorochromes - Marty Bigos

Multicolor IF analysis-advantages of multiple lasers - Bob Hoffman

Compensation in the FACSDiVa - Ben Verwer

TUTORIAL: Simplified and clarified exposition on multicolor fluorescence and compensation including autofluorescence effects - Dave Parks

Theoretical and experimental results for triggering rates and sorting rates in flow - Bob Hoffman

Sorting tradeoff and performance in the FACSDiVa with zero dead-time - Ben Verwer

Event coincidence problem in high-speed cell sorting - Ger van den Engh

Problems inherent in Sorting for Stem Cell Re-engraftment - Alan Fisher

FCML markup language to express experiment level semantic description - Adam Treister

PERL scripts to perform FCS file administration - Adam Treister

Error in flow cytometry measurements - Ger van den Engh

TUTORIAL: Standardization and calibration for flow - Bob Hoffman

Methods for measuring flow cytometry performance - Bob Hoffman

Predicting resolution of dimly fluorescent particles-theoretical and experimental results - Bob Hoffman

Improving fluorescence sensitivity: where do you get the biggest MESFs for your buck? - Kit Snow

Flow cytometer measurement quality comparisons:  test sample set development, analysis procedure and preliminary results - Dave Parks

Limitations for detecting sub-micron particles - Bob Hoffman

TUTORIAL: Cytometry for Microbiology - Howard Shapiro

Evaluation of fluorescent tagging methods for microbes: FISH (rRNA), antibody based, DNA dyes - Kristi Harkins

Immunomagnetic separation of microbes prior to flow detection - Kristi Harkins

Where does FCM fit in the market of rapid detection methods for bacteria? - Kristi Harkins

Nanocrystal probes for flow and imaging - Bill Hyun

Approaches for lower cost flow designs - Bob Hoffman

THE FUTURE - DISCUSSION LEADERS:

Imaging - Jeff Price

Flow - Dave Parks

• Automated High Throughput Microscope Imaging