The most important thing about this program is the times when food and refreshments will be served. These times will not change. The program lists the approximate time a subject will be discussed, although the order of subjects may change at any time at the discretion of the attendees. The length of time spent on any topic will also depend on the interest of the attendees.

5:00-6:00 Session 1 - IMAGE SOFTWARE PART I - Bob Murphy

6:00-7:00 DINNER

7:30-9:00 Session 1, continued

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 2 - FLOW PART I - Ger van den Engh

10:00-10:30 BREAK

10:30-12:00 Session 2, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 3 - IMAGE HARDWARE - Ilya Ravkin

6:00-7:00 DINNER

7:30-9:00 Session 4 - IMAGE SOFTWARE PART II - Damir Sudar

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 Session 5 - FLOW PART II - Bob Auer

10:00-10:30 BREAK

10:30-12:00 Session 5, continued

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 Session 5, continued

6:00-7:00 DINNER

7:30-9:00 Session 5, continued

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

8:30-10:00 BREAK-OUT SESSIONS

10:30-12:00 BREAK-OUT SESSIONS

12:00-1:00 LUNCH

1:00-5:00 FREE TIME

5:00-6:00 BREAK-OUT SESSIONS

6:00-7:00 DINNER

7:30-9:00 BREAK-OUT SESSION REPORTS / THE FUTURE

9:00-10:30 HAPPY HOUR / Informal Discussions

7:30-8:30 BREAKFAST

Departure

** = Tutorial; 15 minutes max.

* = Regular Presentation; 8 minutes max

** Wavelets based biomedical image processing - Yu-Ping Wang

** Multivariate distance and similarity - Bob Murphy

* Statistically-sound interpretation of imaging experiments - Bob Murphy

* Application of spline theory to biomedical image computation - Yu-Ping Wang

* Typical image selection - Bob Murphy

* A large particle sorter (300 Hz, 1 mm orifice) - Peter Nelson

* Use of the InFlux sorter in clone selection - Ger van den Engh

* Automated hematology analysis: erythroblasts - Young-Ran Kim

* Automated hematology analysis: CD61 analysis - Young-Ran Kim

* Automated hematology analysis: CD3/4 and CD3/8 - Young-Ran Kim

* Aspects of high-sensitivity instrumentation: optics, data acquisition, performance - Rob Habbersett

** Use of LED’s as light sources and measurement tools in flow cytometry - Bob Hoffman

* Update on UV/violet/blue/green solid-state lasers - Howard Shapiro

* Results with Nichia violet diode laser - Bob Hoffman

* Update on detectors - Howard Shapiro

* Optical filter selection for flow cytometry - John Sharpe

* Drosophila: Imaging an entire organism at cellular resolution - David Knowles

* Direct control of micromirrors - Mark Corio

* Fluorescence Imaged Microdeformation: A rheological and imaging technique unravels membrane function - David Knowles

* Web-based microscope - Steve Kelley

* A limited survey of high resolution CCD cameras - Damir Sudar

* Annotation requirements for image data bases - Bob Murphy

* Image Informatics - Ilya Ravkin

* Classification of subcellular localization patterns in 3D - Meel Velliste

* Patterns of Proteins: Model based image analysis to link in vivo protein distribution and cell phenotype - David Knowles

* Making protein localization features more portable - Meel Velliste

* Optical DNA fiber mapping- Damir Sudar

* Fetal cell screening using maternal blood - Fatima Merchant

* Image processing in microvolume laser scanning cytometry (MLSC) - Scott Norton

* A microfabricated cell sorter with integrated valves and pumps - Anne Fu

* Velocity-independent microfluidic flow cytometry - Shuli Eyal

** Digital data acquisition systems - Bob Auer

* Digital pulse processing vs. high-resolution digitization of held pulses - Nancy Perlmutter

* Digital data acquisition - Bob Hoffman

* High resolution data acquisition and how to test it - Howard Shapiro

* Multicolor compensation models - Marty Bigos

* Fluorescence spectral compensation - Jim Wood

* Effect of spectral overlap on reducing fluorescence sensitivity - Bob Hoffman

* Quantitative modeling of results - Bob Hoffman

* Polarization is dependent on dye concentration - Jeanne Uy

* Certifying fluorescent particle CV’s - Bob Hoffman

* Predicting performance of fluorescence analysis - Bob Hoffman

* Measurement of flow cytometer light collection efficiency and background noise - Jim Wood

* High speed sorting - Bob Hoffman

* Sorter illumination and monitoring - Dick Stovel

* FCM data presentation in log vs. linear displays - Jim Wood

* BNF description of FCS file format - Jim Wood

• Inexpensive Cytometers for the Third World
• Image Databases
• Flow Cytometry Standard Practices