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Murphy Lab - Protocols - Flow Cytometry - Acidification


Measuring pH of endocytic compartments

Detailed protocols for monitoring acidification of endocytosed probes using flow cytometry may be found in Sheree Lynn Rybak and Robert F. Murphy (1997) "Measurement of Ligand Acidification Kinetics for Adherent and Non-Adherent Cells" in In Living Color -- Protocols in Flow Cytometry and Cell Sorting (Rochelle Diamond and Susan L. DeMaggio, eds.), R.G. Landes Co., in press. The following spreadsheets (in different formats) are referred to in that chapter and are provided to illustrate how dual-fluorescence measurements are converted to estimates of pH:




Last Updated: 01 Dec 2004




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