,/2 H   d .Chart 1  @ @?pH curve for A549 Cells using DextranpHFITC/Cy5$&|Example sheet for protocol 4a%*'Ref: Sheree Lynn Rybak and Robert F. Murphy, Measurement of Ligand Acidification Kinetics for Adherent and Non-Adherent Cellst'In: In Living Color -- Protocols in Flow Cytometry and Cell Sorting (Rochelle Diamond and Sue DeMaggio, eds.)'R.G. Landes Co., 1997O'Calibration curve for pH measurements using FITC-dextran and Cy5-dextran 'Cells: ' A549 "pH'Labeled?"Mean FITC"Mean Cy5"Avg FITC"Avg Cy5 "pH"FITC-bkg "Cy5-bkg "FITC/Cy5 "Norm to pH 7.24 @ 'Yes ףp= @@ Gzi@|?5=@ Q|?u@ Q @96@   /i@   qh3L?   GU%lA?   @  'Yes  M"B@  \(9@     |?5:F@   B`"~@   ^h?   ;D:?   @  'Yes  t0@  Gzxz@     ROb@    -~@    r'[v?   B\?   @  'Yes  Mb(@@  d;O;@     @ rh|f@   gffff|@   (L?   4̲?   @  'No  X9v>@  v:3@ On@ Q ^I 3@ Q  (\@ ClSl@   7A`%@   I!25?   ?   @  'No  fffff@  Zd3@     "y_p@   "0@   ȥ%?   "20V?    'Yes +NM@ zGE@H@ Qx&@ Q  'Yes %CB@ ףp= y@    'Yes Mb0G@ F}@    'Yes Cl K@  +Ђ@    'No  r@ ̌1@B`"@ QK7A0@ Q  'No M@ Cl,@    'Yes `"ۥ`@  y@x&b@ QHz@ Q  'Yes Qe@ /$T@    'Yes Mb4a@ QC@    'Yes h|?c@ n‚@    'No Mb@ E42@Cl@ QCl1@ Q  'No X9v>@ A`1@   @ 'Yes +g@ L7A`z@1g@ Q.輪}@ Q @ 'Yes - l@ x&@   @ 'Yes y&1g@ sh|'|@   @ 'Yes L7A`-a@ ~jt{@   @ 'No "~@ Dli*@(\@ Qx&1/@ Q @ 'No NbX94@ 33333S2@   (\@  'Yes  SKq@  )\ς@ /$m@ Q vo@ Q !(\@ !'Yes !;Op@ !x}@ ! ! "(\@ "'Yes "B`"h@ "V-ł@ " " #(\@ #'Yes #)\i@ #33333)|@ # # $(\@ $'No $Hz@ $jtd2@$ףp= W@ Q$K7 1@ Q %(\@ %'No %fffff@ %&1\/@ % % & &'Yes &NbX9n@ &H@&p= Wp@ Q&~j@ Q ' ''Yes '|?5:t@ '?5^IԂ@ ' ' ( ('Yes (?5^I2p@ (jt@ ( ( ) )'Yes )/$m@ )zG|@ ) ) * *'No *ffffff@ *%C +@*K7@ Q*p= ף+@ Q + +'No +x&1@ +t,@ + +,'Signal:Noise (pH 8),$K@  ,UE@