Murphy Lab

 Cytometry Development Workshop

 Flow Cytometry



 Carnegie Mellon University
 Computational Biology Department
 Center for Bioimage Informatics
 Biological Sciences Department
 Biomedical Engineering Department
 Machine Learning Department

SImEC - Statistical Imaging Experiment Comparator

Objective evaluation of differences in protein subcellular distribution
Edward J.S. Roques and Robert F. Murphy
Department of Biological Sciences, Biomedical Engineering Program, and Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh PA 15213/USA


In order to compare a change in a protein's subcellular localization following a change in expression or in environmental conditions it is first necessary to obtain image sets of the initial and final distribution. Visual analysis of these image sets may prove inadequate for quantitative statements regarding a change in protein localization. SImEC performs a quantitative analysis of the image sets and returns a degree of similarity between the two distributions and whether the protein localization can be regarded as being from the same class, within a statistical level of confidence.


Previous work from our laboratory has described a method for the numerical description of protein localization according to three sets of features: Zernike moment features, Haralick texture features, and features specifically derived for analysis of subcellular location. These features have been shown to be capable of distinguishing the major subcellular organelles and structures of cultured cells. These features can be combined in different ways to form different Subcellular Location Feature (SLF) sets.


HeLa cell images showing the distributions of the Golgi proteins giantin and gpp130 that were collected as part of our previous classification project were used to illustrate the SImEC service. Parallel images showing the distributions of DNA were used, along with hand-specified polygonal regions that isolated an individual cell in each image. Over 80 images for each protein were processed by SImEC using the SLF6 feature set with a confidence level of 0.05. The results generated by SImEC are available here. Examples of the images are shown below.
Protein Image
DNA Image
Crop Image

Last Updated: 01 Dec 2004

Copyright © 1996-2016 by the Murphy Lab, Carnegie Mellon University