Cytometry Development Workshop
Movies and Maps of protein distributions during T cell signallingThis page contains raw data and computationally-derived maps from the manuscript
K. T. Roybal, T. E. Buck, X. Ruan, B. H. Cho, D. J. Clark, R. Ambler, H. M. Tunbridge, J. Zhang, P. Verkade, C. Wülfing, and R. F. Murphy (2016) Automated spatiotemporal analysis identifies WAVE2 and Cofilin as joint regulators of costimulation-mediated T cell actin dynamics. Science Signaling 9:rs3. DOI: 10.1126/scisignal.aad4149
Movies consist of frames of DIC and fluorescence containing fields of T cells expressing an eGFP-tagged protein and antigen-presenting cells expressing the appropriate antigen. Frames were taken at various times, and the time and position of synapse formation has been manually annotated. The movies and annotations files are are grouped into ZIP files for each tagged protein. The ZIP files range in size from 1 GB to 8 GB.
The annotations that accompany these movies, marking the locations and times of synapse formations, are contained in Excel spreadsheets in this zip file: Tcell_annotations.zip.
The ZIP file below contains maps for both the average values and the standard deviations across all cells for each sensor, time point and condition. Maps are contained in separate files for each time point and consist of fluorescence values for each voxel of a 3D template. Within each ZIP file is a folder containing images (scaled to the maximum average value for a given map) and a folder containing comma-separated value (csv) files containing the relative fluorescence intensity values (scaled to a fraction of the total fluorescence). Also included is a csv file containing the average cellular concentration of each probe (except MRLC). A Matlab function that can read the csv files and return 3D images either as relative fluorescence or concentration values is also available.
Please contact Bob Murphy for questions or help.
Last Updated: 19 Apr 2016
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