Supplementary material for Automated Image Analysis of Protein Localization in Budding Yeast List of 501 proteins whose label from visual assignment differs from that by automated classification. ORF UCSF localization Automated localization Confidence YAL034W-A spindle_pole nucleus 100.00% YBL002W nucleus nucleolus 100.00% YBL003C nucleus nucleolus 100.00% YBL010C late_Golgi cytoplasm 100.00% YBL031W microtubule vacuole 100.00% YBR096W ER cytoplasm 100.00% YBR141C nucleolus nucleus 100.00% YBR211C spindle_pole nucleus 100.00% YCR048W ER cytoplasm 100.00% YCR067C ER cytoplasm 100.00% YCR087C-A nucleolus nucleus 100.00% YDL100C ER cytoplasm 100.00% YDR026C nucleolus endosome 100.00% YDR125C mitochondrion cytoplasm 100.00% YDR170C late_Golgi mitochondrion 100.00% YDR208W cytoplasm ER 100.00% YDR224C nucleus nucleolus 100.00% YDR265W peroxisome mitochondrion 100.00% YDR284C vacuolar_membrane vacuole 100.00% YDR329C peroxisome mitochondrion 100.00% YDR407C late_Golgi mitochondrion 100.00% YFL025C ER cytoplasm 100.00% YFR019W vacuolar_membrane cytoplasm 100.00% YGL047W ER cytoplasm 100.00% YGL083W late_Golgi mitochondrion 100.00% YGL153W peroxisome mitochondrion 100.00% YGR014W vacuole nucleus 100.00% YGR080W actin mitochondrion 100.00% YGR101W mitochondrion vacuole 100.00% YGR133W peroxisome mitochondrion 100.00% YGR279C vacuole nucleus 100.00% YGR283C nucleolus nucleus 100.00% YHL018W mitochondrion cytoplasm 100.00% YHR072W lipid_particle mitochondrion 100.00% YHR098C ER_to_Golgi ER 100.00% YHR108W late_Golgi mitochondrion 100.00% YIL044C late_Golgi ER 100.00% YIL147C cell_periphery cytoplasm 100.00% YJL044C late_Golgi cytoplasm 100.00% YJL079C vacuole nucleus 100.00% YJL081C nucleus nucleolus 100.00% YJL091C ER cytoplasm 100.00% YJL151C vacuole nucleus 100.00% YJR118C ER cytoplasm 100.00% YKL007W actin mitochondrion 100.00% YKL078W nucleolus nucleus 100.00% YKL119C ER cytoplasm 100.00% YKL179C lipid_particle ER 100.00% YKL185W nucleus vacuole 100.00% YKL205W nuclear_periphery vacuole 100.00% YKR046C lipid_particle mitochondrion 100.00% YLR001C vacuolar_membrane vacuole 100.00% YLR088W ER cytoplasm 100.00% YLR100W lipid_particle mitochondrion 100.00% YLR110C ER cytoplasm 100.00% YLR141W nucleolus nucleus 100.00% YLR206W actin cytoplasm 100.00% YLR247C nucleus vacuole 100.00% YLR268W Golgi cytoplasm 100.00% YLR330W late_Golgi mitochondrion 100.00% YLR429W actin mitochondrion 100.00% YML008C lipid_particle mitochondrion 100.00% YML069W nucleus nucleolus 100.00% YML121W vacuolar_membrane ER 100.00% YMR004W endosome mitochondrion 100.00% YMR052W ER cytoplasm 100.00% YMR106C nucleus vacuole 100.00% YMR129W nuclear_periphery vacuole 100.00% YMR148W lipid_particle mitochondrion 100.00% YMR310C nucleus mitochondrion 100.00% YNL008C nuclear_periphery vacuole 100.00% YNL106C actin mitochondrion 100.00% YNL169C mitochondrion ER 100.00% YNL214W peroxisome mitochondrion 100.00% YNL231C lipid_particle ER 100.00% YNL299W nucleolus vacuole 100.00% YNR004W nucleolus nucleus 100.00% YNR047W cytoplasm cell_periphery 100.00% YOL011W vacuole ER 100.00% YOL088C vacuole ER 100.00% YOL147C peroxisome mitochondrion 100.00% YOL154W vacuole cytoplasm 100.00% YOR033C nucleus vacuole 100.00% YOR193W peroxisome mitochondrion 100.00% YOR214C ER cytoplasm 100.00% YOR249C nucleus vacuole 100.00% YOR311C ER cytoplasm 100.00% YOR377W ER cytoplasm 100.00% YPL051W early_Golgi mitochondrion 100.00% YPL085W ER_to_Golgi ER 100.00% YPL116W bud_neck cytoplasm 100.00% YPL162C vacuolar_membrane vacuole 100.00% YPR075C vacuole ER 100.00% YPR128C peroxisome mitochondrion 100.00% YPR140W mitochondrion cytoplasm 100.00% YPR171W actin mitochondrion 100.00% YBR207W vacuolar_membrane vacuole 96.00% YCR088W actin mitochondrion 96.00% YDL161W actin ER 96.00% YDR421W nucleus vacuole 96.00% YDR439W nucleolus nucleus 96.00% YER124C bud_neck vacuole 96.00% YFR046C nucleus mitochondrion 96.00% YGL206C late_Golgi mitochondrion 96.00% YGL247W nuclear_periphery vacuole 96.00% YGR026W cell_periphery ER 96.00% YGR266W ER cell_periphery 96.00% YHL017W late_Golgi mitochondrion 96.00% YIL047C vacuolar_membrane vacuole 96.00% YIL109C ER_to_Golgi ER 96.00% YJL004C early_Golgi mitochondrion 96.00% YJL062W ER vacuole 96.00% YJL207C late_Golgi mitochondrion 96.00% YJL210W peroxisome mitochondrion 96.00% YJR054W vacuole cytoplasm 96.00% YKR068C late_Golgi endosome 96.00% YLL014W ER cytoplasm 96.00% YLL055W ER vacuole 96.00% YLR023C ER cytoplasm 96.00% YLR051C nucleolus nucleus 96.00% YLR083C vacuole cytoplasm 96.00% YML010W nucleus nucleolus 96.00% YMR092C actin cytoplasm 96.00% YMR198W microtubule vacuole 96.00% YMR218C late_Golgi mitochondrion 96.00% YNL095C ER vacuole 96.00% YNL198C mitochondrion cytoplasm 96.00% YNL211C mitochondrion vacuole 96.00% YOL048C lipid_particle mitochondrion 96.00% YOR037W mitochondrion cytoplasm 96.00% YOR291W ER cytoplasm 96.00% YPL207W ER cell_periphery 96.00% YPR090W late_Golgi cytoplasm 96.00% YAL031C cytoplasm ER 92.00% YBL013W mitochondrion cytoplasm 92.00% YDL173W cytoplasm cell_periphery 92.00% YDR201W spindle_pole mitochondrion 92.00% YDR210W cell_periphery cytoplasm 92.00% YDR424C ER cytoplasm 92.00% YDR451C nucleus vacuole 92.00% YGL098W lipid_particle mitochondrion 92.00% YGL186C ER vacuole 92.00% YGR043C nucleus vacuole 92.00% YGR247W late_Golgi mitochondrion 92.00% YHL008C vacuole ER 92.00% YHL039W cytoplasm peroxisome 92.00% YHR060W ER cytoplasm 92.00% YHR079C ER cytoplasm 92.00% YIL173W endosome cytoplasm 92.00% YJL131C mitochondrion vacuole 92.00% YKL004W early_Golgi mitochondrion 92.00% YKR035W-A endosome nucleolus 92.00% YLL003W spindle_pole mitochondrion 92.00% YLL012W lipid_particle mitochondrion 92.00% YLR034C ER cytoplasm 92.00% YLR066W ER cytoplasm 92.00% YLR080W vacuole cytoplasm 92.00% YLR126C cytoplasm vacuole 92.00% YLR211C cytoplasm vacuole 92.00% YLR219W cell_periphery ER 92.00% YLR324W peroxisome mitochondrion 92.00% YLR342W vacuole ER 92.00% YLR363C cytoplasm ER 92.00% YLR451W nucleus vacuole 92.00% YML049C cytoplasm vacuole 92.00% YMR160W vacuolar_membrane vacuole 92.00% YNL083W mitochondrion cytoplasm 92.00% YNL142W cell_periphery cytoplasm 92.00% YNR013C ER cytoplasm 92.00% YOR104W cell_periphery ER 92.00% YOR130C mitochondrion cytoplasm 92.00% YOR246C lipid_particle mitochondrion 92.00% YOR317W ER cytoplasm 92.00% YPL232W lipid_particle vacuole 92.00% YPL259C late_Golgi ER 92.00% YPR008W cytoplasm cell_periphery 92.00% YPR188C bud_neck cytoplasm 92.00% YAL027W nucleus mitochondrion 88.00% YBL102W early_Golgi ER 88.00% YBR127C vacuolar_membrane cytoplasm 88.00% YBR274W cytoplasm mitochondrion 88.00% YCL043C vacuole vacuolar_membrane 88.00% YDL008W vacuolar_membrane cytoplasm 88.00% YDR229W vacuolar_membrane spindle_pole 88.00% YEL036C Golgi late_Golgi 88.00% YER032W bud_neck mitochondrion 88.00% YFR037C nucleus nucleolus 88.00% YGL226C-A ER cytoplasm 88.00% YGR055W vacuole nucleus 88.00% YGR167W late_Golgi mitochondrion 88.00% YIL039W ER cytoplasm 88.00% YJL036W endosome cytoplasm 88.00% YJL117W ER cytoplasm 88.00% YLR396C vacuolar_membrane nucleus 88.00% YNL328C mitochondrion cytoplasm 88.00% YOR058C microtubule cytoplasm 88.00% YOR264W bud_neck vacuole 88.00% YAL002W endosome spindle_pole 84.00% YAR002C-A vacuolar_membrane ER 84.00% YBL059W mitochondrion vacuole 84.00% YBL086C cell_periphery mitochondrion 84.00% YDR046C vacuole ER 84.00% YFL041W vacuolar_membrane nuclear_periphery 84.00% YGR015C mitochondrion cytoplasm 84.00% YGR108W nucleus vacuole 84.00% YGR218W nucleus cytoplasm 84.00% YHR023W bud_neck cytoplasm 84.00% YIL159W bud_neck mitochondrion 84.00% YIL172C cytoplasm vacuole 84.00% YKL073W vacuole cytoplasm 84.00% YLR191W peroxisome mitochondrion 84.00% YLR194C ER cytoplasm 84.00% YLR275W nucleus vacuole 84.00% YLR346C mitochondrion ER 84.00% YLR356W mitochondrion cytoplasm 84.00% YMR111C nucleus vacuole 84.00% YNL046W ER vacuole 84.00% YOR040W mitochondrion cytoplasm 84.00% YOR308C nucleus nucleolus 84.00% YOR357C vacuolar_membrane vacuole 84.00% YPL050C Golgi vacuole 84.00% YBL063W microtubule cytoplasm 80.00% YBR234C actin mitochondrion 80.00% YBR271W cytoplasm vacuole 80.00% YBR275C nucleus vacuole 80.00% YDL001W cytoplasm vacuole 80.00% YDL028C bud_neck cytoplasm 80.00% YDL054C vacuolar_membrane vacuole 80.00% YDR225W vacuole nucleus 80.00% YDR388W actin ER 80.00% YEL048C late_Golgi mitochondrion 80.00% YER011W ER vacuole 80.00% YER019C-A ER late_Golgi 80.00% YER145C cell_periphery cytoplasm 80.00% YGL037C peroxisome mitochondrion 80.00% YHR147C cell_periphery cytoplasm 80.00% YHR192W cytoplasm late_Golgi 80.00% YIL095W actin mitochondrion 80.00% YJR138W vacuolar_membrane ER 80.00% YKL019W vacuole nucleus 80.00% YKL094W ER actin 80.00% YKL208W mitochondrion vacuole 80.00% YKR037C spindle_pole vacuolar_membrane 80.00% YLR283W mitochondrion nucleus 80.00% YMR019W nucleus mitochondrion 80.00% YMR032W bud_neck cytoplasm 80.00% YNL125C ER vacuole 80.00% YNR056C cell_periphery cytoplasm 80.00% YOR299W late_Golgi cytoplasm 80.00% YPL242C bud_neck mitochondrion 80.00% YPL255W spindle_pole endosome 80.00% YDL204W ER mitochondrion 76.00% YDR039C cell_periphery cytoplasm 76.00% YER063W nucleus vacuole 76.00% YFR028C nucleolus spindle_pole 76.00% YGL079W endosome vacuole 76.00% YGR089W ER cell_periphery 76.00% YGR206W endosome cytoplasm 76.00% YJL099W late_Golgi cytoplasm 76.00% YJL157C nucleus vacuole 76.00% YKL002W endosome nucleus 76.00% YKL108W nucleus cytoplasm 76.00% YKR089C lipid_particle mitochondrion 76.00% YLR148W endosome vacuolar_membrane 76.00% YLR408C endosome vacuole 76.00% YMR161W ER cytoplasm 76.00% YMR168C microtubule vacuole 76.00% YMR204C peroxisome mitochondrion 76.00% YNL219C ER nuclear_periphery 76.00% YOR056C cytoplasm ER 76.00% YAL023C ER cytoplasm 72.00% YDL010W vacuole nucleus 72.00% YDL107W mitochondrion vacuole 72.00% YDL170W nucleus vacuole 72.00% YDL226C cytoplasm ER 72.00% YDR014W nucleus vacuole 72.00% YDR153C late_Golgi mitochondrion 72.00% YDR254W microtubule cytoplasm 72.00% YDR275W lipid_particle mitochondrion 72.00% YDR323C endosome mitochondrion 72.00% YER151C cytoplasm ER 72.00% YGL005C Golgi cytoplasm 72.00% YGL032C vacuole cytoplasm 72.00% YGR263C ER cytoplasm 72.00% YHR186C vacuolar_membrane cytoplasm 72.00% YHR194W nucleus vacuole 72.00% YIR003W actin ER 72.00% YJL058C cytoplasm cell_periphery 72.00% YKL012W nucleus peroxisome 72.00% YKL048C bud_neck cytoplasm 72.00% YKR027W late_Golgi mitochondrion 72.00% YLR073C endosome cytoplasm 72.00% YLR315W spindle_pole nucleus 72.00% YLR360W endosome mitochondrion 72.00% YMR022W ER cytoplasm 72.00% YMR182C nucleus vacuole 72.00% YNR039C ER cytoplasm 72.00% YOL023W mitochondrion ER 72.00% YOL126C peroxisome actin 72.00% YPL045W vacuolar_membrane mitochondrion 72.00% YBR067C ER cytoplasm 68.00% YBR138C cytoplasm vacuole 68.00% YCR086W nucleolus nucleus 68.00% YDL022W peroxisome ER 68.00% YDL157C mitochondrion ER 68.00% YDR197W mitochondrion cytoplasm 68.00% YER166W late_Golgi ER 68.00% YGL255W ER cytoplasm 68.00% YHR106W mitochondrion cytoplasm 68.00% YJL201W cytoplasm ER 68.00% YJR003C mitochondrion ER 68.00% YKL197C peroxisome cytoplasm 68.00% YLL015W vacuolar_membrane nucleus 68.00% YLR005W nucleus endosome 68.00% YLR025W endosome spindle_pole 68.00% YLR195C cytoplasm ER 68.00% YLR354C nucleus nucleolus 68.00% YML037C late_Golgi cytoplasm 68.00% YMR179W nucleus cytoplasm 68.00% YMR289W cytoplasm vacuole 68.00% YMR304W cytoplasm ER 68.00% YNL138W actin cell_periphery 68.00% YNL146W ER endosome 68.00% YNL279W vacuole ER 68.00% YOL007C vacuole mitochondrion 68.00% YOR025W nucleus vacuole 68.00% YOR123C nucleus nucleolus 68.00% YOR147W mitochondrion nucleus 68.00% YOR233W cell_periphery cytoplasm 68.00% YPL195W early_Golgi ER 68.00% YPR046W nucleus lipid_particle 68.00% YPR079W endosome vacuolar_membrane 68.00% YAL010C mitochondrion cytoplasm 64.00% YBL025W nucleolus nucleus 64.00% YBL057C mitochondrion cytoplasm 64.00% YDR123C nucleus vacuolar_membrane 64.00% YDR314C nucleus mitochondrion 64.00% YEL059C-A cytoplasm vacuole 64.00% YER047C cytoplasm ER 64.00% YGL006W vacuolar_membrane cytoplasm 64.00% YGL124C late_Golgi endosome 64.00% YHR045W ER cytoplasm 64.00% YHR114W actin ER 64.00% YIL048W early_Golgi ER 64.00% YIL062C actin mitochondrion 64.00% YJL048C nuclear_periphery vacuole 64.00% YJL212C ER vacuole 64.00% YKR031C cytoplasm cell_periphery 64.00% YLR024C cytoplasm mitochondrion 64.00% YLR063W cytoplasm vacuole 64.00% YLR409C nucleus nucleolus 64.00% YML107C nuclear_periphery nucleus 64.00% YMR055C spindle_pole cytoplasm 64.00% YMR195W vacuolar_membrane cytoplasm 64.00% YMR270C nucleolus nucleus 64.00% YNL020C actin peroxisome 64.00% YNL225C spindle_pole mitochondrion 64.00% YOR191W nucleus vacuole 64.00% YOR248W vacuole ER 64.00% YOR296W cytoplasm vacuole 64.00% YPL112C peroxisome mitochondrion 64.00% YPL157W nucleolus vacuole 64.00% YPL158C bud_neck cytoplasm 64.00% YPL209C microtubule nucleus 64.00% YDL195W ER_to_Golgi cell_periphery 60.00% YDL227C nucleus mitochondrion 60.00% YDR079W mitochondrion cytoplasm 60.00% YDR137W early_Golgi late_Golgi 60.00% YDR291W nucleus vacuole 60.00% YDR320C-A spindle_pole nucleolus 60.00% YDR503C Golgi cytoplasm 60.00% YHR164C nucleus vacuole 60.00% YKL042W spindle_pole mitochondrion 60.00% YLL052C ER cytoplasm 60.00% YML011C nucleus vacuole 60.00% YNL041C Golgi mitochondrion 60.00% YOL043C mitochondrion vacuole 60.00% YOR046C nuclear_periphery ER 60.00% YBL075C cytoplasm bud_neck 56.00% YBR302C vacuole cytoplasm 56.00% YDR437W ER cytoplasm 56.00% YER044C ER cytoplasm 56.00% YER075C cytoplasm vacuole 56.00% YGL010W ER cytoplasm 56.00% YGL093W spindle_pole mitochondrion 56.00% YGL223C early_Golgi mitochondrion 56.00% YHR110W ER cytoplasm 56.00% YIL066C cytoplasm vacuole 56.00% YJL153C cytoplasm vacuole 56.00% YJR075W Golgi mitochondrion 56.00% YKL146W ER cell_periphery 56.00% YLR208W ER_to_Golgi ER 56.00% YML059C ER cytoplasm 56.00% YML081W nucleus vacuole 56.00% YMR090W cytoplasm bud_neck 56.00% YMR246W lipid_particle ER 56.00% YNL094W cytoplasm mitochondrion 56.00% YNL194C cell_periphery vacuole 56.00% YNR035C actin mitochondrion 56.00% YOL144W nucleolus nucleus 56.00% YOR002W ER cytoplasm 56.00% YPL076W ER cytoplasm 56.00% YPL223C cytoplasm vacuole 56.00% YAL009W nucleus vacuole 52.00% YAL058W ER cytoplasm 52.00% YBR222C cytoplasm ER 52.00% YBR254C late_Golgi ER 52.00% YBR296C ER vacuole 52.00% YCR043C Golgi late_Golgi 52.00% YDL027C mitochondrion cell_periphery 52.00% YDR128W vacuolar_membrane vacuole 52.00% YDR221W ER cytoplasm 52.00% YDR304C vacuole nucleus 52.00% YDR381W nucleus nucleolus 52.00% YER053C-A ER cytoplasm 52.00% YER064C nucleus vacuole 52.00% YGL086W nuclear_periphery vacuole 52.00% YGR295C vacuole nucleus 52.00% YHL014C mitochondrion vacuole 52.00% YIL117C vacuole nucleus 52.00% YJL025W nucleolus nucleus 52.00% YJR143C ER cytoplasm 52.00% YKL013C actin late_Golgi 52.00% YKR010C nucleolus nucleus 52.00% YKR039W vacuole vacuolar_membrane 52.00% YKR087C mitochondrion vacuole 52.00% YLR370C actin cytoplasm 52.00% YML034W nuclear_periphery nucleus 52.00% YML038C early_Golgi late_Golgi 52.00% YMR173W cytoplasm ER 52.00% YNL003C mitochondrion vacuole 52.00% YNL049C ER_to_Golgi cytoplasm 52.00% YNL230C nucleus mitochondrion 52.00% YNL267W cytoplasm vacuole 52.00% YNR075W ER cytoplasm 52.00% YOL013C ER mitochondrion 52.00% YOL072W nuclear_periphery vacuolar_membrane 52.00% YOL101C ER vacuole 52.00% YOL116W microtubule vacuole 52.00% YPL234C ER cytoplasm 52.00% YPR184W cytoplasm late_Golgi 52.00% YBR041W lipid_particle ER 48.00% YBR230C mitochondrion vacuole 48.00% YCL005W lipid_particle vacuole 48.00% YDR006C nucleus vacuolar_membrane 48.00% YDR227W nuclear_periphery vacuolar_membrane 48.00% YEL043W ER cell_periphery 48.00% YER016W microtubule spindle_pole 48.00% YER098W cytoplasm mitochondrion 48.00% YER123W vacuole cytoplasm 48.00% YHR015W cytoplasm peroxisome 48.00% YLR204W mitochondrion cytoplasm 48.00% YLR259C mitochondrion peroxisome 48.00% YLR289W mitochondrion vacuole 48.00% YLR381W spindle_pole vacuole 48.00% YML109W cytoplasm cell_periphery 48.00% YML128C ER nuclear_periphery 48.00% YMR266W cell_periphery ER 48.00% YBR042C lipid_particle vacuole 44.00% YDR062W ER actin 44.00% YDR100W early_Golgi late_Golgi 44.00% YGL059W mitochondrion ER 44.00% YGL145W ER cytoplasm 44.00% YGL216W microtubule nucleus 44.00% YGR239C peroxisome vacuole 44.00% YHR120W mitochondrion nucleolus 44.00% YJL183W Golgi cytoplasm 44.00% YJR031C Golgi ER 44.00% YJR127C nucleus mitochondrion 44.00% YMR071C late_Golgi ER 44.00% YNR006W endosome vacuole 44.00% YOR091W cytoplasm mitochondrion 44.00% YDL193W ER Golgi 40.00% YDR246W late_Golgi ER 40.00% YHR190W ER mitochondrion 40.00% YKL063C Golgi ER 40.00% YKL089W spindle_pole vacuole 40.00% YKL135C late_Golgi mitochondrion 40.00% YKL165C vacuole cytoplasm 40.00% YLR240W endosome vacuolar_membrane 40.00% YMR066W mitochondrion nuclear_periphery 40.00% YMR171C endosome mitochondrion 40.00% YNR031C cytoplasm vacuole 40.00% YOR099W vacuole ER 40.00% YOR350C mitochondrion endosome 40.00% YOR367W actin cytoplasm 40.00% YPL006W vacuolar_membrane ER 40.00% YPL020C nuclear_periphery ER 40.00% YPL180W vacuolar_membrane endosome 40.00% YPR105C early_Golgi cell_periphery 40.00% YDR245W Golgi ER 36.00% YEL013W vacuolar_membrane nucleus 36.00% YGL038C Golgi vacuole 36.00% YJR092W bud_neck endosome 36.00% YLR042C ER mitochondrion 36.00% YOR321W ER cytoplasm 36.00% YBR015C Golgi vacuole 32.00% YDR464W nucleus mitochondrion 32.00% YOR081C lipid_particle mitochondrion 32.00% YPL252C mitochondrion cytoplasm 32.00% YCL014W bud_neck mitochondrion 28.00%